total ulk1 (Cell Signaling Technology Inc)
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Structured Review
Cell Signaling Technology Inc
total ulk1
Total Ulk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+ulk1+antibody/Phospho-ULK1+(Ser757)+Antibody/pmc04041039-76-113-115
Average 96 stars, based on 640 article reviews
Total Ulk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 640 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/total+ulk1+antibody/Phospho-ULK1+(Ser757)+Antibody/pmc04041039-76-113-115
Average 96 stars, based on 640 article reviews
total ulk1 - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "Regulation of ubiquitin-proteasome and autophagy pathways after acute LPS and epoxomicin administration in mice"
Article Title: Regulation of ubiquitin-proteasome and autophagy pathways after acute LPS and epoxomicin administration in mice
Journal: BMC Musculoskeletal Disorders
doi: 10.1186/1471-2474-15-166
Figure Legend Snippet: Effect of LPS on autophagy protein markers and lysosomal enzymes. Protein expression level of LC3bII (A) , protein expression ratio of LC3aII on LC3aI (B) , protein expression level of p62 (C) , phosphorylation state of ULK1 Ser757 (D) , protein expression level of ULK1 (E) , enzymatic activities of Cathepsin B (F) and Cathepsin L (G) in the GAS of mice 8 hours after administration of LPS 7.5 mg/kg with or without epoxomicin 0.5 mg/kg treatment. Values are means ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared with control (C) .
Techniques Used: Expressing
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LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Recombinant:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Activity Assay:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Microscopy:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Two Tailed Test:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Infection:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Blocking Assay:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Standard Deviation:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: shRNA:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Selection:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Staining:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: De-Phosphorylation Assay:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Inhibition:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Activation Assay:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: Incubation:Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr172), total AMPK, phospho-ULK1 (Ser555), total ULK1, phosph-Beclin-1 (Ser93), total Beclin-1, phospho-IKK (Ser176/180), total IKKb, and LC3A/B, Article Title: mTOR independent regulation of macroautophagy by Leucine Rich Repeat Kinase 2 via Beclin-1 Article Snippet: Antibodies used were as follows: LC3 antibody (NB100-2220, Novus Biologicals); LRRK2 antibodies (MJFF#2, 3514-1/ab133474, Epitomics); total Article Title: The effect of different acute muscle contraction regimens on the expression of muscle proteolytic signaling proteins and genes Article Snippet: After blocking, the membranes were washed and incubated overnight at 4°C with primary antibodies, including p‐AMPK α (Thr172, cat#2531), total AMPK (cat#2532), p‐ULK1 (Ser317, cat#12753), p‐ULK1 (Thr757, cat#14202), total ULK1 (cat#8054), LC3B (cat#2775), p‐FoxO3a (Ser253, cat#9466), total FoxO3a (cat#2497), Article Title: High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells Article Snippet: The kinase reaction was stopped by the addition of Laemmli buffer 2×, and the samples were then heated for 5 min at 95 °C, loaded onto SDS-PAGE, transferred to a nitrocellulose membrane, and probed with the Article Title: Cell adhesion molecule IGPR-1 activates AMPK connecting cell adhesion to autophagy Article Snippet: Phospho-AMPK (Thr 172 ), total AMPK, phospho-ULK1 (Ser 555 ), total ULK1, phosph-Beclin-1 (Ser 93 ), total Beclin-1, phospho-IKK (Ser 176/180 ), total IKKβ, and LC3A/B, Article Title: Modulation of Autophagy by a Small Molecule Inverse Agonist of ERRα Is Neuroprotective Article Snippet: |